A P Das
Siksha O Anusandhan University, India
Title: Biosensors for rapid detection of endotoxins in pharmaceutical drugs and biological fluids
Biography
Biography: A P Das
Abstract
Endotoxin control is of paramount importance primarily for health and safety issues. There are many sectors demanding mandatory monitoring of endotoxin levels, such as food industry, pharmaceutical, health care, agriculture, and utilities, defense and the environment. Mortality and morbidity rates due to endotoxin remain paramount high and have not improved significantly over the past 25 years. The outcome of failure to detect and monitor endotoxin levels can result in serious sequelae in survivors and large scale deaths. These necessities the need for a rapid, sensitive and non-invasive biosensor for detection and monitoring of endotoxin levels even in extremely small concentrations. The proposed method of endotoxin detection is based on the use Chromogenic substrate, which monitors, the color development of the Horseshoe crab amoebocyte lysate assay in response to endotoxin. Our proposed strategy can be adapted to detect endotoxin detection in pharmaceutical drugs and biological fluids with 0.02EU/ml. Due to its rapidity, portability and sensitivity the proposed chromogenic sensor is ideally suited for wide-spread use in endotoxin detection.